Dataset Information

Higher expression of several interferon-stimulated genes in HIV-1-infected females after adjusting for the level of viral replication.

ABSTRACT:

Background

Clinical studies have shown faster disease progression and stronger immune activation in human immunodeficiency virus (HIV)-1-infected females when compared with males for the same level of HIV-1 replication. Here we determine whether the elevated levels of HIV-1-induced interferon-alpha (IFN-?) production observed in females are associated with higher interferon-stimulated gene (ISG) expression levels in T cells, hence suggesting type-I IFN as a mechanism for the higher HIV-1-associated immune activation observed.

Methods

T-cell and dendritic cell populations were isolated from treatment-naive chronically HIV-1-infected individuals enrolled in the Adult Clinical Trials Group 384 by fluorescence-activated cell sorting. The expression of 98 genes involved in Toll-like receptor and type I IFN signaling pathways were quantified using Nanostring technology.

Results

Several ISGs were significantly correlated with HIV-1 viral load and/or CD4(+) T-cell count. Higher expression levels of a subset of these ISGs were observed in cells derived from females as compared to males after adjusting for viral load and were correlated to higher levels of T-cell activation.

Conclusion

These data show that higher IFN-? production is associated with higher ex vivo expression of several ISGs in females. This might contribute to higher levels of immune activation and the observed faster HIV-1 disease progression in females for a given level of viral replication.

SUBMITTER: Chang JJ

PROVIDER: S-EPMC3733517 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Publications

Higher expression of several interferon-stimulated genes in HIV-1-infected females after adjusting for the level of viral replication.

Chang J Judy JJ Woods Matt M Lindsay Robert J RJ Doyle Erin H EH Griesbeck Morgane M Chan Ellen S ES Robbins Gregory K GK Bosch Ronald J RJ Altfeld Marcus M

The Journal of infectious diseases 20130610 5

<h4>Background</h4>Clinical studies have shown faster disease progression and stronger immune activation in human immunodeficiency virus (HIV)-1-infected females when compared with males for the same level of HIV-1 replication. Here we determine whether the elevated levels of HIV-1-induced interferon-alpha (IFN-α) production observed in females are associated with higher interferon-stimulated gene (ISG) expression levels in T cells, hence suggesting type-I IFN as a mechanism for the higher HIV-1 ...[more]

PMID: 23757341

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Dataset Information

Higher expression of several interferon-stimulated genes in HIV-1-infected females after adjusting for the level of viral replication.

Background

Methods

Results

Conclusion

Publications

Higher expression of several interferon-stimulated genes in HIV-1-infected females after adjusting for the level of viral replication.

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