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Mass recalibration of FT-ICR mass spectrometry imaging data using the average frequency shift of ambient ions.

ABSTRACT: Achieving and maintaining high mass measurement accuracy (MMA) throughout a mass spectrometry imaging (MSI) experiment is vital to the identification of the observed ions. However, when using FTMS instruments, fluctuations in the total ion abundance at each pixel due to inherent biological variation in the tissue section can introduce space charge effects that systematically shift the observed mass. Herein we apply a recalibration based on the observed cyclotron frequency shift of ions found in the ambient laboratory environment, polydimethylcyclosiloxanes (PDMS). This calibration method is capable of achieving part per billion (ppb) mass accuracy with relatively high precision for an infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) MSI dataset. Comparisons with previously published mass calibration approaches are also presented.

SUBMITTER: Barry JA

PROVIDER: S-EPMC3739293 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Mass recalibration of FT-ICR mass spectrometry imaging data using the average frequency shift of ambient ions.

Barry Jeremy A JA Robichaud Guillaume G Muddiman David C DC

Journal of the American Society for Mass Spectrometry 20130529 7

Achieving and maintaining high mass measurement accuracy (MMA) throughout a mass spectrometry imaging (MSI) experiment is vital to the identification of the observed ions. However, when using FTMS instruments, fluctuations in the total ion abundance at each pixel due to inherent biological variation in the tissue section can introduce space charge effects that systematically shift the observed mass. Herein we apply a recalibration based on the observed cyclotron frequency shift of ions found in ...[more]

PMID: 23715870

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