Ontology highlight
ABSTRACT:
SUBMITTER: Karatekin E
PROVIDER: S-EPMC3747733 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Karatekin Erdem E Rothman James E JE
Nature protocols 20120419 5
Many biological processes rely on membrane fusion, and therefore assays to study its mechanisms are necessary. Here we report an assay with sensitivity to single-vesicle, and even to single-molecule events using fluorescently labeled vesicle-associated v-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) liposomes and target-membrane-associated t-SNARE-reconstituted planar, supported bilayers (t-SBLs). Docking and fusion events can be detected using conventional far-fi ...[more]