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Imaging bacterial protein expression using genetically encoded RNA sensors.

ABSTRACT: The difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome by using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins and exhibit fluorescence increases that enable protein expression to be imaged in living Escherichia coli. These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time.

SUBMITTER: Song W

PROVIDER: S-EPMC3758421 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Imaging bacterial protein expression using genetically encoded RNA sensors.

Song Wenjiao W Strack Rita L RL Jaffrey Samie R SR

Nature methods 20130721 9

The difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome by using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins and exhibit fluorescence increases that enable protein expression to be imaged in living Escherichia coli. These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time. ...[more]

PMID: 23872791

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