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Genetically encoded RNA-based sensors for intracellular imaging of silver ions.

ABSTRACT: Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag+ in live bacterial cells. The sensor is designed by introducing a cytosine-Ag+-cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag+ induces the folding of Broccoli and activates a fluorescence signal. This sensor can be genetically encoded to measure the cellular flux and antibacterial effect of Ag+.

SUBMITTER: Yu Q

PROVIDER: S-EPMC6328328 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Genetically encoded RNA-based sensors for intracellular imaging of silver ions.

Yu Qikun Q Shi Jing J Mudiyanselage Aruni P K K Karunanayake APKKK Wu Rigumula R Zhao Bin B Zhou Ming M You Mingxu M

Chemical communications (Cambridge, England) 20190101 5

Silver has been widely used for disinfection. The cellular accumulation of silver ions (Ag+) is critical in these antibacterial effects. The direct cellular measurement of Ag+ is useful for the study of disinfection mechanisms. Herein, we reported a novel genetically encoded RNA-based sensor to image Ag+ in live bacterial cells. The sensor is designed by introducing a cytosine-Ag+-cytosine metallo base pair into a fluorogenic RNA aptamer, Broccoli. The binding of Ag+ induces the folding of Brocc ...[more]

PMID: 30566125

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