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Engineering genetically encoded FRET sensors.

ABSTRACT: Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor fluorescent protein that occur when an attached sensor domain undergoes a change in conformation in response to ligand binding. The design of sensitive FRET sensors remains challenging as there are few generally applicable design rules and each sensor must be optimized anew. In this review we discuss various strategies that address this shortcoming, including rational design approaches that exploit self-associating fluorescent domains and the directed evolution of FRET sensors using high-throughput screening.

SUBMITTER: Lindenburg L

PROVIDER: S-EPMC4168480 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Engineering genetically encoded FRET sensors.

Lindenburg Laurens L Merkx Maarten M

Sensors (Basel, Switzerland) 20140702 7

Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor fluorescent protein that occur when an attached sensor domain undergoes a change in conformation in response to ligand binding. The design of sensitive FRET sensors remains challenging as there are few generally applicable design rules and each sensor ...[more]

PMID: 24991940

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