Ontology highlight
ABSTRACT:
SUBMITTER: Lindert S
PROVIDER: S-EPMC3760413 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Lindert Steffen S Staritzbichler René R Wötzel Nils N Karakaş Mert M Stewart Phoebe L PL Meiler Jens J
Structure (London, England : 1993) 20090701 7
In medium-resolution (7-10 A) cryo-electron microscopy (cryo-EM) density maps, alpha helices can be identified as density rods whereas beta-strand or loop regions are not as easily discerned. We are proposing a computational protein structure prediction algorithm "EM-Fold" that resolves the density rod connectivity ambiguity by placing predicted alpha helices into the density rods and adding missing backbone coordinates in loop regions. In a benchmark of 11 mainly alpha-helical proteins of known ...[more]