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Androgen receptor agonism promotes an osteogenic gene program in preadipocytes.

ABSTRACT: Androgens regulate body composition by interacting with the androgen receptor (AR) to control gene expression in a tissue-specific manner. To identify novel regulatory roles for AR in preadipocytes, we created a 3T3-L1 cell line stably expressing human AR. We found AR expression is required for androgen-mediated inhibition of 3T3-L1 adipogenesis. This inhibition is characterized by decreased lipid accumulation, reduced expression of adipogenic genes, and induction of genes associated with osteoblast differentiation. Collectively, our results suggest androgens promote an osteogenic gene program at the expense of adipocyte differentiation.

SUBMITTER: Hartig SM

PROVIDER: S-EPMC3763992 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Androgen receptor agonism promotes an osteogenic gene program in preadipocytes.

Hartig Sean M SM Feng Qin Q Ochsner Scott A SA Xiao Rui R McKenna Neil J NJ McGuire Sean E SE He Bin B

Biochemical and biophysical research communications 20130406 2

Androgens regulate body composition by interacting with the androgen receptor (AR) to control gene expression in a tissue-specific manner. To identify novel regulatory roles for AR in preadipocytes, we created a 3T3-L1 cell line stably expressing human AR. We found AR expression is required for androgen-mediated inhibition of 3T3-L1 adipogenesis. This inhibition is characterized by decreased lipid accumulation, reduced expression of adipogenic genes, and induction of genes associated with osteob ...[more]

PMID: 23567971

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