Burst activity and ultrafast activation kinetics of CaV1.3 Ca²? channels support presynaptic activity in adult gerbil hair cell ribbon synapses.

ABSTRACT: Auditory information transfer to afferent neurons relies on precise triggering of neurotransmitter release at the inner hair cell (IHC) ribbon synapses by Ca²? entry through CaV1.3 Ca²? channels. Despite the crucial role of CaV1.3 Ca²? channels in governing synaptic vesicle fusion, their elementary properties in adult mammals remain unknown. Using near-physiological recording conditions we investigated Ca²? channel activity in adult gerbil IHCs. We found that Ca²? channels are partially active at the IHC resting membrane potential (-60 mV). At -20 mV, the large majority (>70%) of Ca²? channel first openings occurred with an estimated delay of about 50 ?s in physiological conditions, with a mean open time of 0.5 ms. Similar to other ribbon synapses, Ca²? channels in IHCs showed a low mean open probability (0.21 at -20 mV), but this increased significantly (up to 0.91) when Ca²? channel activity switched to a bursting modality. We propose that IHC Ca²? channels are sufficiently rapid to transmit fast signals of sound onset and support phase-locking. Short-latency Ca²? channel opening coupled to multivesicular release would ensure precise and reliable signal transmission at the IHC ribbon synapse.

SUBMITTER: Zampini V 

PROVIDER: S-EPMC3764630 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature