Ontology highlight
ABSTRACT:
SUBMITTER: Cymer F
PROVIDER: S-EPMC3767533 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Cymer Florian F von Heijne Gunnar G
Proceedings of the National Academy of Sciences of the United States of America 20130819 36
Polytopic membrane proteins are inserted cotranslationally into target membranes by ribosome-translocon complexes. It is, however, unclear when during the insertion process specific interactions between the transmembrane helices start to form. Here, we use a recently developed in vivo technique to measure pulling forces acting on transmembrane helices during their cotranslational insertion into the inner membrane of Escherichia coli to study the earliest steps of tertiary folding of five polytop ...[more]