Ontology highlight
ABSTRACT:
SUBMITTER: Gilbert LA
PROVIDER: S-EPMC3770145 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Cell 20130711 2
The genetic interrogation and reprogramming of cells requires methods for robust and precise targeting of genes for expression or repression. The CRISPR-associated catalytically inactive dCas9 protein offers a general platform for RNA-guided DNA targeting. Here, we show that fusion of dCas9 to effector domains with distinct regulatory functions enables stable and efficient transcriptional repression or activation in human and yeast cells, with the site of delivery determined solely by a coexpres ...[more]