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Biomimetic antigenic nanoparticles elicit controlled protective immune response to influenza.

ABSTRACT: Here we present a biomimetic strategy toward nanoparticle design for controlled immune response through encapsulation of conserved internal influenza proteins on the interior of virus-like particles (VLPs) to direct CD8+ cytotoxic T cell protection. Programmed encapsulation and sequestration of the conserved nucleoprotein (NP) from influenza on the interior of a VLP, derived from the bacteriophage P22, results in a vaccine that provides multistrain protection against 100 times lethal doses of influenza in an NP specific CD8+ T cell-dependent manner. VLP assembly and encapsulation of the immunogenic NP cargo protein is the result of a genetically programmed self-assembly making this strategy amendable to the quick production of vaccines to rapidly emerging pathogens. Addition of adjuvants or targeting molecules were not required for eliciting the protective response.

SUBMITTER: Patterson DP

PROVIDER: S-EPMC3773536 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Biomimetic antigenic nanoparticles elicit controlled protective immune response to influenza.

Patterson Dustin P DP Rynda-Apple Agnieszka A Harmsen Ann L AL Harmsen Allen G AG Douglas Trevor T

ACS nano 20130410 4

Here we present a biomimetic strategy toward nanoparticle design for controlled immune response through encapsulation of conserved internal influenza proteins on the interior of virus-like particles (VLPs) to direct CD8+ cytotoxic T cell protection. Programmed encapsulation and sequestration of the conserved nucleoprotein (NP) from influenza on the interior of a VLP, derived from the bacteriophage P22, results in a vaccine that provides multistrain protection against 100 times lethal doses of in ...[more]

PMID: 23540530

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