Ontology highlight
ABSTRACT:
SUBMITTER: Richards AL
PROVIDER: S-EPMC6434932 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Richards Alicia L AL Hebert Alexander S AS Ulbrich Arne A Bailey Derek J DJ Coughlin Emma E EE Westphall Michael S MS Coon Joshua J JJ
Nature protocols 20150409 5
Recent advances in chromatography and mass spectrometry (MS) have made rapid and deep proteomic profiling possible. To maximize the performance of the recently produced Orbitrap hybrid mass spectrometer, we have developed a protocol that combines improved sample preparation (including optimized cellular lysis by extensive bead beating) and chromatographic conditions (specifically, 30-cm capillary columns packed with 1.7-μm bridged ethylene hybrid material) and the manufacture of a column heater ...[more]