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Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer.

ABSTRACT: Colorectal cancer (CRC) is an important disorder that results from genetic and epigenetic alterations in one colonic epithelial cell. Epidermal growth factor (EGF) is critical in the development of tumors in epithelial tissues. Variations in the DNA sequence of the EGF gene may be particularly significant with regard to susceptibility to CRC. The present study aimed to investigate the effect of the EGF gene single nucleotide polymorphism (SNP), rs2298979, on CRC. In this prospective study, 220 samples were collected from patients with CRC and compared with 220 matched healthy controls. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and the result was validated by direct sequencing. A significant correlation was observed between the rs2298979 variant in the EGF gene and CRC. The frequency of the A/G genotype in the control group was higher than in the patients with sporadic CRC [odds ratio (OR), 0.488; 95% confidence interval (CI), 0.307-0.774; P=0.002]. In this study there were no individuals with a G/G genotype. Although the frequency of the G and A alleles was similar in the healthy control and CRC patient groups, individuals with the A/G genotype were less susceptible to CRC compared with those with the A/A genotype.

SUBMITTER: Chaleshi V

PROVIDER: S-EPMC3796412 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer.

Chaleshi Vahid V Haghighi Mahdi Montazer MM Savabkar Sanaz S Zali Neda N Vahedi Mohsen M Khanyaghma Mahsa M Javadi Gholam Reza GR Asadzade Hamid H Zali Mohammad Reza MR

Oncology letters 20130722 4

Colorectal cancer (CRC) is an important disorder that results from genetic and epigenetic alterations in one colonic epithelial cell. Epidermal growth factor (EGF) is critical in the development of tumors in epithelial tissues. Variations in the DNA sequence of the <i>EGF</i> gene may be particularly significant with regard to susceptibility to CRC. The present study aimed to investigate the effect of the <i>EGF</i> gene single nucleotide polymorphism (SNP), rs2298979, on CRC. In this prospectiv ...[more]

PMID: 24137467

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Project description:Objective: To study the relationship between long-chain non-coding RNA small nucleolar RNA host gene 16 (lncRNA SNHG16) polymorphisms and its interaction with environmental factors and susceptibility to colorectal cancer (CRC).Methods: Sanger sequencing was used to analyze genotypes of lncRNA SNHG16 gene rs7353, rs8038, and rs15278 sites. Multifactor dimensionality reduction was used to analyze interactions between lncRNA SNHG16 gene rs7353, rs8038, rs15278 sites, and environmental factors. Haploview 4.1 software was used to analyze linkage disequilibrium of lncRNA SNHG16 gene rs7353, rs8038, and rs15278 sites. Quantitative real-time polymerase chain reaction was used to analyze plasma lncRNA SNHG16 levels of CRC patients and control subjects.Results: Variation of the lncRNA SNHG16 gene rs7353 site A>G variation was associated with decreased CRC susceptibility (Odds ratio [OR]?=?0.50, 95% confidence interval [CI]: 0.40-0.62, P?<?.01). The rs8038 site G>A and rs15278 site A>G variation were associated with increased CRC susceptibility (OR?=?1.87, 95% CI: 1.47-2.36, P?<?.01). The rs15278 site G>A variation was associated with increased CRC susceptibility (OR?=?2.24, 95% CI: 1.61-3.11, P?<?.01). Interaction combinations featuring age, rs7353, rs8038, and rs15278 single nucleotide polymorphism are 13.53 times more susceptible to CRC than other interactions (95% CI: 9.43-19.41, P?<?.01). The rs15278, rs8038, and rs7353 site AGA haplotypes were significantly associated with a decreased CRC risk (OR?=?0.65, 95% CI: 0.48-0.88, P?=?.01), AAG haplotypes were significantly associated with an increased CRC risk (OR?=?2.00, 95% CI: 1.27-3.17, P?<?.01). High lncRNA SNHG16 expression was associated with tumor progression in CRC patients (??=?8.85, P?=?.03). The rs7353 site A>G variation caused a significant decrease in plasma lncRNA SNHG16 level (P?<?.01), while the rs8038 site G>A variation and rs15278 site A>G variation resulted in increased plasma lncRNA SNHG16 levels.Conclusion: Polymorphisms of lncRNA SNHG16 gene rs7353, rs8038, rs15278 loci and their interaction with age are significantly associated with CRC susceptibility.

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Dataset Information

Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer.

Publications

Correlation between the EGF gene intronic polymorphism, rs2298979, and colorectal cancer.

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