Ontology highlight
ABSTRACT:
SUBMITTER: Ringel AE
PROVIDER: S-EPMC3798266 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Ringel Alison E AE Ryznar Rebecca R Picariello Hannah H Huang Kuan-lin KL Lazarus Asmitha G AG Holmes Scott G SG
PLoS genetics 20131017 10
Sir2 is an NAD(+)-dependent histone deacetylase required to mediate transcriptional silencing and suppress rDNA recombination in budding yeast. We previously identified Tdh3, a glyceraldehyde 3-phosphate dehydrogenase (GAPDH), as a high expression suppressor of the lethality caused by Sir2 overexpression in yeast cells. Here we show that Tdh3 interacts with Sir2, localizes to silent chromatin in a Sir2-dependent manner, and promotes normal silencing at the telomere and rDNA. Characterization of ...[more]