Epigenetic modifications induced by Blimp-1 Regulate CD8? T cell memory progression during acute virus infection.

ABSTRACT: The transcription factor Blimp-1 regulates the overall accumulation of virus-specific CD8? T cells during acute viral infections. We found that increased proliferation and survival of Blimp-1-deficient CD8? T cells resulted from sustained expression of CD25 and CD27 and persistent cytokine responsiveness. Silencing of Il2ra and Cd27 reduced the Blimp-1-deficient CD8? T cell response. Genome-wide chromatin immunoprecipitation (ChIP) sequencing analysis identified Il2ra and Cd27 as direct targets of Blimp-1. At the peak of the antiviral response, but not earlier, Blimp-1 recruited the histone-modifying enzymes G9a and HDAC2 to the Il2ra and Cd27 loci, thereby repressing expression of these genes. In the absence of Blimp-1, Il2ra and Cd27 exhibited enhanced histone H3 acetylation and reduced histone H3K9 trimethylation. These data elucidate a central mechanism by which Blimp-1 acts as an epigenetic regulator and enhances the numbers of short-lived effector cells while suppressing the development of memory-precursor CD8? T cells.

SUBMITTER: Shin HM 

PROVIDER: S-EPMC3808842 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature