Ontology highlight
ABSTRACT:
SUBMITTER: Codreanu SG
PROVIDER: S-EPMC3811082 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Codreanu Simona G SG Kim Hye-Young H HY Porter Ned A NA Liebler Daniel C DC
Methods in molecular biology (Clifton, N.J.) 20120101
Formation of covalent protein adducts by lipid electrophiles contributes to diseases and toxicities linked to oxidative stress, but analysis of the adducts presents a challenging analytical problem. We describe selective adduct capture using biotin affinity probes to enrich protein and peptide adducts for analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). One approach employs biotinamidohexanoic acid hydrazide to covalently label residual carbonyl groups on adducts. The other ...[more]