Ontology highlight
ABSTRACT:
SUBMITTER: Codreanu SG
PROVIDER: S-EPMC3811082 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Methods in molecular biology (Clifton, N.J.) 20120101
Formation of covalent protein adducts by lipid electrophiles contributes to diseases and toxicities linked to oxidative stress, but analysis of the adducts presents a challenging analytical problem. We describe selective adduct capture using biotin affinity probes to enrich protein and peptide adducts for analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). One approach employs biotinamidohexanoic acid hydrazide to covalently label residual carbonyl groups on adducts. The other ...[more]