Ontology highlight
ABSTRACT:
SUBMITTER: Beavers WN
PROVIDER: S-EPMC6174696 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Beavers William N WN Rose Kristie L KL Galligan James J JJ Mitchener Michelle M MM Rouzer Carol A CA Tallman Keri A KA Lamberson Connor R CR Wang Xiaojing X Hill Salisha S Ivanova Pavlina T PT Brown H Alex HA Zhang Bing B Porter Ned A NA Marnett Lawrence J LJ
ACS chemical biology 20170628 8
Determining the impact of lipid electrophile-mediated protein damage that occurs during oxidative stress requires a comprehensive analysis of electrophile targets adducted under pathophysiological conditions. Incorporation of ω-alkynyl linoleic acid into the phospholipids of macrophages prior to activation by Kdo<sub>2</sub>-lipid A, followed by protein extraction, click chemistry, and streptavidin affinity capture, enabled a systems-level survey of proteins adducted by lipid electrophiles gener ...[more]