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Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.


ABSTRACT: Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection.

SUBMITTER: O'Connell KJ 

PROVIDER: S-EPMC3813531 | biostudies-literature | 2013 Sep-Oct

REPOSITORIES: biostudies-literature

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Identification and characterization of an oleate hydratase-encoding gene from Bifidobacterium breve.

O'Connell Kerry Joan KJ   Motherway Mary O'Connell MO   Hennessey Alan A AA   Brodhun Florian F   Ross R Paul RP   Feussner Ivo I   Stanton Catherine C   Fitzgerald Gerald F GF   van Sinderen Douwe D  

Bioengineered 20130829 5


Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2  ...[more]

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