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Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.

ABSTRACT: Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289?K using a solution consisting of 0.1?M Tris-HCl pH 8.0, 0.2?M LiSO4, 22%(w/v) PEG 3350. X-ray diffraction data were collected to 2.8?Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c = 120.53?Å, ? = 88.37, ? = 78.77, ? = 82.65°.

SUBMITTER: Wen J

PROVIDER: S-EPMC3818039 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4.

Wen Jinjin J Li Zhenzhen Z He Guangzheng G Xu Shujing S Zhao Baohua B Zhu Xianming X Dong Hui H Ju Jiansong J

Acta crystallographica. Section F, Structural biology and crystallization communications 20131017 Pt 11

Alanine dehydrogenase (OF4Ald) from the alkaliphilic Bacillus pseudofirmus OF4 was expressed and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M Tris-HCl pH 8.0, 0.2 M LiSO4, 22%(w/v) PEG 3350. X-ray diffraction data were collected to 2.8 Å resolution. The crystal belonged to the triclinic space group P1, with unit-cell parameters a = 88.04, b = 105.59, c ...[more]

PMID: 24192355

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