Ontology highlight
ABSTRACT:
SUBMITTER: Wei W
PROVIDER: S-EPMC4094479 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Wei Wei W Xin Huhu H Roy Bhaskar B Dai Junbiao J Miao Yungen Y Gao Guanjun G
PloS one 20140711 7
We report the establishment of an efficient and heritable gene mutagenesis method in the silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with an associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, and Bmtan as candidates, we proved that genome alterations at specific sites could be induced by direct microinjection of specific guide RNA and Cas9-mRNA into silkworm embryos. Mutation frequencies of 16.7-35.0% were obse ...[more]