Ontology highlight
ABSTRACT:
SUBMITTER: Liu ML
PROVIDER: S-EPMC3843951 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Liu Meng-Lu ML Zang Tong T Zou Yuhua Y Chang Joshua C JC Gibson Jay R JR Huber Kimberly M KM Zhang Chun-Li CL
Nature communications 20130101
Cell fate can be reprogrammed by modifying intrinsic and extrinsic cues. Here we show that two small molecules (forskolin and dorsomorphin) enable the transcription factor Neurogenin 2 (NGN2) to convert human fetal lung fibroblasts into cholinergic neurons with high purity (>90%) and efficiency (up to 99% of NGN2-expressing cells). The conversion is direct without passing through a proliferative progenitor state. These human induced cholinergic neurons (hiCN) show mature electrophysiological pro ...[more]