Ontology highlight
ABSTRACT:
SUBMITTER: Koh EY
PROVIDER: S-EPMC3857217 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Koh Esther Y C EY Ho Steven C L SC Mariati Song Zhiwei Z Bi Xuezhi X Bardor Muriel M Yang Yuansheng Y
PloS one 20131209 12
A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) elements with varying strengths is generated by mutating the translation initiation codons of 10(th), 11(th), and 12(th) AUG to non-AUG triplets. They are able to control the relative expression of multiple genes over a wide range in mammalian cells in both transient and stable transfections. The relative strength of each IRES mutant remains similar in different mammalian cell lines and is not gene specific. T ...[more]