TARP ?-7 selectively enhances synaptic expression of calcium-permeable AMPARs.

ABSTRACT: Regulation of calcium-permeable AMPA receptors (CP-AMPARs) is crucial in normal synaptic function and neurological disease states. Although transmembrane AMPAR regulatory proteins (TARPs) such as stargazin (?-2) modulate the properties of calcium-impermeable AMPARs (CI-AMPARs) and promote their synaptic targeting, the TARP-specific rules governing CP-AMPAR synaptic trafficking remain unclear. We used RNA interference to manipulate AMPAR-subunit and TARP expression in ?-2-lacking stargazer cerebellar granule cells--the classic model of TARP deficiency. We found that TARP ?-7 selectively enhanced the synaptic expression of CP-AMPARs and suppressed CI-AMPARs, identifying a pivotal role of ?-7 in regulating the prevalence of CP-AMPARs. In the absence of associated TARPs, both CP-AMPARs and CI-AMPARs were able to localize to synapses and mediate transmission, although their properties were altered. Our results also establish that TARPed synaptic receptors in granule cells require both ?-2 and ?-7 and reveal an unexpected basis for the loss of AMPAR-mediated transmission in stargazer mice.

SUBMITTER: Studniarczyk D 

PROVIDER: S-EPMC3858651 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature