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Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.


ABSTRACT: We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.

SUBMITTER: Martin L 

PROVIDER: S-EPMC3863600 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Martin Lance L   Meier Matthias M   Lyons Shawn M SM   Sit Rene V RV   Marzluff William F WF   Quake Stephen R SR   Chang Howard Y HY  

Nature methods 20121111 12


We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretati  ...[more]

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