Ontology highlight
ABSTRACT:
SUBMITTER: Martin L
PROVIDER: S-EPMC3863600 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Martin Lance L Meier Matthias M Lyons Shawn M SM Sit Rene V RV Marzluff William F WF Quake Stephen R SR Chang Howard Y HY
Nature methods 20121111 12
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretati ...[more]