Ontology highlight
ABSTRACT:
SUBMITTER: Corrigan AM
PROVIDER: S-EPMC3865054 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Cell cycle (Georgetown, Tex.) 20130716 16
Spindle orientation defines the plane of cell division and, thereby, the spatial position of all daughter cells. Here, we develop a live cell microscopy-based methodology to extract spindle movements in human epithelial cell lines and study how spindles are brought to a pre-defined orientation. We show that spindles undergo two distinct regimes of movements. Spindles are first actively rotated toward the cells' long-axis and then maintained along this pre-defined axis. By quantifying spindle mov ...[more]