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DNA polymerases ? and Rev1 mediate error-prone bypass of non-B DNA structures.


ABSTRACT: DNA polymerase ? (Pol ?) and Rev1 are key players in translesion DNA synthesis. The error-prone Pol ? can also participate in replication of undamaged DNA when the normal replisome is impaired. Here we define the nature of the replication disturbances that trigger the recruitment of error-prone polymerases in the absence of DNA damage and describe the specific roles of Rev1 and Pol ? in handling these disturbances. We show that Pol ?/Rev1-dependent mutations occur at sites of replication stalling at short repeated sequences capable of forming hairpin structures. The Rev1 deoxycytidyl transferase can take over the stalled replicative polymerase and incorporate an additional 'C' at the hairpin base. Full hairpin bypass often involves template-switching DNA synthesis, subsequent realignment generating multiply mismatched primer termini and extension of these termini by Pol ?. The postreplicative pathway dependent on polyubiquitylation of proliferating cell nuclear antigen provides a backup mechanism for accurate bypass of these sequences that is primarily used when the Pol ?/Rev1-dependent pathway is inactive. The results emphasize the pivotal role of noncanonical DNA structures in mutagenesis and reveal the long-sought-after mechanism of complex mutations that represent a unique signature of Pol ?.

SUBMITTER: Northam MR 

PROVIDER: S-EPMC3874155 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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DNA polymerases ζ and Rev1 mediate error-prone bypass of non-B DNA structures.

Northam Matthew R MR   Moore Elizabeth A EA   Mertz Tony M TM   Binz Sara K SK   Stith Carrie M CM   Stepchenkova Elena I EI   Wendt Kathern L KL   Burgers Peter M J PM   Shcherbakova Polina V PV  

Nucleic acids research 20130918 1


DNA polymerase ζ (Pol ζ) and Rev1 are key players in translesion DNA synthesis. The error-prone Pol ζ can also participate in replication of undamaged DNA when the normal replisome is impaired. Here we define the nature of the replication disturbances that trigger the recruitment of error-prone polymerases in the absence of DNA damage and describe the specific roles of Rev1 and Pol ζ in handling these disturbances. We show that Pol ζ/Rev1-dependent mutations occur at sites of replication stallin  ...[more]

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