Unknown

Dataset Information

0

Important functional roles of basigin in thymocyte development and T cell activation.


ABSTRACT: Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we generated a conditional null allele by introducing two loxP sites flanking exons 2 and 7 of the basigin gene (Bsg). Bsg(fl/fl) mice were born at the expected Mendelian ratio and showed a similar growth rate compared with wildtype mice. After crossing these mice with Lck-Cre transgenic mice, basigin expression was specifically inactivated in T cells in the resulting Lck-Cre; Bsg(fl/fl) mice. Although the birth and growth rate of Lck-Cre; Bsg(fl/fl) mice were similar to control mice, thymus development was partially arrested in Lck-Cre; Bsg(fl/fl) mice, specifically at the CD4(+)CD8(+) double-positive (DP) and CD4 single-positive (CD4(+)CD8(-), CD4SP) stages. In addition, CD4(+) T cell activation was enhanced upon Concanavalin A (Con A) or anti-CD3/anti-CD28 stimulation but not upon PMA/Ionomycin stimulation in the absence of basigin. Overall, this study provided the first in vivo evidence for the function of basigin in thymus development. Moreover, the successful generation of the conditional null basigin allele provides a useful tool for the study of distinct physiological or pathological functions of basigin in different tissues at different development stages.

SUBMITTER: Yao H 

PROVIDER: S-EPMC3879590 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications

Important functional roles of basigin in thymocyte development and T cell activation.

Yao Hui H   Teng Yan Y   Sun Qian Q   Xu Jing J   Chen Ya-Tong YT   Hou Ning N   Cheng Xuan X   Yang Xiao X   Chen Zhi-Nan ZN  

International journal of biological sciences 20131213 1


Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we generated a conditional null allele by introducing two loxP sites flanking exons 2 and 7 of the basigin gene (Bsg). Bsg(fl/fl) mice were born at the expected Mendelian ratio and showe  ...[more]

Similar Datasets

| S-EPMC2848698 | biostudies-literature
| S-EPMC2118636 | biostudies-literature
| S-EPMC2440618 | biostudies-literature
| S-EPMC2848029 | biostudies-literature
| S-EPMC8284395 | biostudies-literature
| S-EPMC7500101 | biostudies-literature
| S-EPMC4490789 | biostudies-literature
| S-EPMC5549601 | biostudies-literature
2013-12-31 | E-GEOD-39946 | biostudies-arrayexpress
| S-EPMC7502710 | biostudies-literature