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Measuring local-directional resolution and local anisotropy in cryo-EM maps.

ABSTRACT: The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.

SUBMITTER: Vilas JL

PROVIDER: S-EPMC6940361 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Measuring local-directional resolution and local anisotropy in cryo-EM maps.

Vilas Jose Luis JL Tagare Hemant D HD Vargas Javier J Carazo Jose Maria JM Sorzano Carlos Oscar S COS

Nature communications 20200102 1

The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. M ...[more]

PMID: 31896756

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