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Extended protocol and real-time PCR dataset for shrimp species identification.

ABSTRACT: Mitochondrial gene analysis was performed using gene-specific conserved primers by conventional and real-time PCR method. Along with, beta-actin gene analysis using conserved primers was also performed in this article. Mitochondrial gene sequence as well as beta-actin sequence data were acquired by Sanger sequencing technique. We have reported a set of conserved primers for the identification and authentication of ten different shrimp species. Real-time melt curve analysis was the most promising methodology to discriminate the mislabeling or fraudulent commercial use of shrimp species. Dataset in this article provided novel insight into identification and authentication of shrimp species by real-time melt curve analysis.

SUBMITTER: Mondal D

PROVIDER: S-EPMC6953599 | biostudies-literature |

REPOSITORIES: biostudies-literature

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