Ontology highlight
ABSTRACT:
SUBMITTER: Gao Q
PROVIDER: S-EPMC3911457 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Gao Qi Q Yun Bingling B Wang Qi Q Jiang Lili L Zhu Haibo H Gao Yanni Y Qin Liting L Wang Yongqiang Y Qi Xiaole X Gao Honglei H Wang Xiaomei X Gao Yulong Y
Journal of clinical microbiology 20131016 1
Avian leukosis virus (ALV) subgroups A, B, and J are very common in poultry flocks and have caused serious economic losses in recent years. A multiplex PCR (mPCR) method for the detection of these three subgroups was developed and optimized in this study. We first designed a common forward primer, PF, and three downstream primers, AR, BR, and JR, which can amplify 715 bp for subgroup A, 515 bp for subgroup B, and 422 bp for subgroup J simultaneously in one reaction. The mPCR method produced neit ...[more]