Ontology highlight
ABSTRACT:
SUBMITTER: Yamanaka K
PROVIDER: S-EPMC3918841 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Yamanaka Kazuya K Reynolds Kirk A KA Kersten Roland D RD Ryan Katherine S KS Gonzalez David J DJ Nizet Victor V Dorrestein Pieter C PC Moore Bradley S BS
Proceedings of the National Academy of Sciences of the United States of America 20140121 5
Recent developments in next-generation sequencing technologies have brought recognition of microbial genomes as a rich resource for novel natural product discovery. However, owing to the scarcity of efficient procedures to connect genes to molecules, only a small fraction of secondary metabolomes have been investigated to date. Transformation-associated recombination (TAR) cloning takes advantage of the natural in vivo homologous recombination of Saccharomyces cerevisiae to directly capture larg ...[more]