Ontology highlight
ABSTRACT:
SUBMITTER: Radke MB
PROVIDER: S-EPMC3920478 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Radke Michael B MB Taft Manuel H MH Stapel Britta B Hilfiker-Kleiner Denise D Preller Matthias M Manstein Dietmar J DJ
eLife 20140211
The small molecule EMD 57033 has been shown to stimulate the actomyosin ATPase activity and contractility of myofilaments. Here, we show that EMD 57033 binds to an allosteric pocket in the myosin motor domain. EMD 57033-binding protects myosin against heat stress and thermal denaturation. In the presence of EMD 57033, ATP hydrolysis, coupling between actin and nucleotide binding sites, and actin affinity in the presence of ATP are increased more than 10-fold. Addition of EMD 57033 to heat-inacti ...[more]