Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.
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ABSTRACT:
SUBMITTER: Winardhi RS
PROVIDER: S-EPMC5153558 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Publications
Probing Small Molecule Binding to Unfolded Polyprotein Based on its Elasticity and Refolding.
Winardhi Ricksen S RS Tang Qingnan Q Chen Jin J Yao Mingxi M Yan Jie J
Biophysical journal 20161201 11
Unfolded protein, a disordered structure found before folding of newly synthesized protein or after protein denaturation, is a substrate for binding by many cellular factors such as heat-stable proteins, chaperones, and many small molecules. However, it is challenging to directly probe such interactions in physiological solution conditions because proteins are largely in their folded state. In this work we probed small molecule binding to mechanically unfolded polyprotein using sodium dodecyl su ...[more]
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