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Short RNA indicator sequences are not completely degraded by autoclaving.

ABSTRACT: Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation methodology should be employed, which may include further autoclaving.

SUBMITTER: Unnithan VV

PROVIDER: S-EPMC3921633 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Short RNA indicator sequences are not completely degraded by autoclaving.

Unnithan Veena V VV Unc Adrian A Joe Valerisa V Smith Geoffrey B GB

Scientific reports 20140212

Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation methodology should be employed, which may include further autoclaving. ...[more]

PMID: 24518856

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