Ontology highlight
ABSTRACT:
SUBMITTER: Kiro R
PROVIDER: S-EPMC3929423 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
RNA biology 20140122 1
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system has recently been used to engineer genomes of various organisms, but surprisingly, not those of bacteriophages (phages). Here we present a method to genetically engineer the Escherichia coli phage T7 using the type I-E CRISPR-Cas system. T7 phage genome is edited by homologous recombination with a DNA sequence flanked by sequences homologous to the desired location. Non-edited genomes are target ...[more]