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Identification of truncated forms of U1 snRNA reveals a novel RNA degradation pathway during snRNP biogenesis.


ABSTRACT: The U1 small nuclear ribonucleoprotein (snRNP) plays pivotal roles in pre-mRNA splicing and in regulating mRNA length and isoform expression; however, the mechanism of U1 snRNA quality control remains undetermined. Here, we describe a novel surveillance pathway for U1 snRNP biogenesis. Mass spectrometry-based RNA analysis showed that a small population of SMN complexes contains truncated forms of U1 snRNA (U1-tfs) lacking the Sm-binding site and stem loop 4 but containing a 7-monomethylguanosine 5' cap and a methylated first adenosine base. U1-tfs form a unique SMN complex, are shunted to processing bodies and have a turnover rate faster than that of mature U1 snRNA. U1-tfs are formed partly from the transcripts of U1 genes and partly from those lacking the 3' box elements or having defective SL4 coding regions. We propose that U1 snRNP biogenesis is under strict quality control: U1 transcripts are surveyed at the 3'-terminal region and U1-tfs are diverted from the normal U1 snRNP biogenesis pathway.

SUBMITTER: Ishikawa H 

PROVIDER: S-EPMC3936765 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Identification of truncated forms of U1 snRNA reveals a novel RNA degradation pathway during snRNP biogenesis.

Ishikawa Hideaki H   Nobe Yuko Y   Izumikawa Keiichi K   Yoshikawa Harunori H   Miyazawa Naoki N   Terukina Goro G   Kurokawa Natsuki N   Taoka Masato M   Yamauchi Yoshio Y   Nakayama Hiroshi H   Isobe Toshiaki T   Takahashi Nobuhiro N  

Nucleic acids research 20131205 4


The U1 small nuclear ribonucleoprotein (snRNP) plays pivotal roles in pre-mRNA splicing and in regulating mRNA length and isoform expression; however, the mechanism of U1 snRNA quality control remains undetermined. Here, we describe a novel surveillance pathway for U1 snRNP biogenesis. Mass spectrometry-based RNA analysis showed that a small population of SMN complexes contains truncated forms of U1 snRNA (U1-tfs) lacking the Sm-binding site and stem loop 4 but containing a 7-monomethylguanosine  ...[more]

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