Ontology highlight
ABSTRACT:
SUBMITTER: Lee YS
PROVIDER: S-EPMC3939873 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Lee Young-Sam YS Gregory Mark T MT Yang Wei W
Proceedings of the National Academy of Sciences of the United States of America 20140121 8
DNA polymerase ζ (Pol ζ) is a eukaryotic B-family DNA polymerase that specializes in translesion synthesis and is essential for normal embryogenesis. At a minimum, Pol ζ consists of a catalytic subunit Rev3 and an accessory subunit Rev7. Mammalian Rev3 contains >3,000 residues and is twice as large as the yeast homolog. To date, no vertebrate Pol ζ has been purified for biochemical characterization. Here we report purification of a series of human Rev3 deletion constructs expressed in HEK293 cel ...[more]