On the GFP-based analysis of dynamic concentration profiles.
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ABSTRACT:
SUBMITTER: Berezhkovskii AM
PROVIDER: S-EPMC3945030 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
On the GFP-based analysis of dynamic concentration profiles.
Berezhkovskii Alexander M AM Shvartsman Stanislav Y SY
Biophysical journal 20140201 3
Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation. ...[more]
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