Dataset Information

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer.

ABSTRACT:

Introduction

Mena, an Ena/VASP protein family member, is a key actin regulatory protein. Mena is up-regulated in breast cancers and promotes invasion and motility of tumor cells. Mena has multiple splice variants, including Mena invasive (MenaINV) and Mena11a, which are expressed in invasive or non-invasive tumor cells, respectively. We developed a multiplex quantitative immunofluorescence (MQIF) approach to assess the fraction of Mena lacking 11a sequence as a method to infer the presence of invasive tumor cells represented as total Mena minus Mena11a (called Menacalc) and determined its association with metastasis in breast cancer.

Methods

The MQIF method was applied to two independent primary breast cancer cohorts (Cohort 1 with 501 and Cohort 2 with 296 patients) using antibodies against Mena and its isoform, Mena11a. Menacalc was determined for each patient and assessed for association with risk of disease-specific death.

Results

Total Mena or Mena11a isoform expression failed to show any statistically significant association with outcome in either cohort. However, assessment of Menacalc showed that relatively high levels of this biomarker is associated with poor outcome in two independent breast cancer cohorts (log rank P = 0.0004 for Cohort 1 and 0.0321 for Cohort 2). Multivariate analysis on combined cohorts revealed that high Menacalc is associated with poor outcome, independent of age, node status, receptor status and tumor size.

Conclusions

High Menacalc levels identify a subgroup of breast cancer patients with poor disease-specific survival, suggesting that Menacalc may serve as a biomarker for metastasis.

SUBMITTER: Agarwal S

PROVIDER: S-EPMC3962029 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Publications

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer.

Agarwal Seema S Gertler Frank B FB Balsamo Michele M Condeelis John S JS Camp Robert L RL Xue Xiaonan X Lin Juan J Rohan Thomas E TE Rimm David L DL

Breast cancer research : BCR 20120912 5

<h4>Introduction</h4>Mena, an Ena/VASP protein family member, is a key actin regulatory protein. Mena is up-regulated in breast cancers and promotes invasion and motility of tumor cells. Mena has multiple splice variants, including Mena invasive (MenaINV) and Mena11a, which are expressed in invasive or non-invasive tumor cells, respectively. We developed a multiplex quantitative immunofluorescence (MQIF) approach to assess the fraction of Mena lacking 11a sequence as a method to infer the presen ...[more]

PMID: 22971274

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Dataset Information

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer.

Introduction

Methods

Results

Conclusions

Publications

Quantitative assessment of invasive mena isoforms (Menacalc) as an independent prognostic marker in breast cancer.

Similar Datasets

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