Ontology highlight
ABSTRACT:
SUBMITTER: Rhoads TW
PROVIDER: S-EPMC3983007 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Rhoads Timothy W TW Rose Christopher M CM Bailey Derek J DJ Riley Nicholas M NM Molden Rosalynn C RC Nestler Amelia J AJ Merrill Anna E AE Smith Lloyd M LM Hebert Alexander S AS Westphall Michael S MS Pagliarini David J DJ Garcia Benjamin A BA Coon Joshua J JJ
Analytical chemistry 20140219 5
The ability to acquire highly accurate quantitative data is an increasingly important part of any proteomics experiment, whether shotgun or top-down approaches are used. We recently developed a quantitation strategy for peptides based on neutron encoding, or NeuCode SILAC, which uses closely spaced heavy isotope-labeled amino acids and high-resolution mass spectrometry to provide quantitative data. We reasoned that the strategy would also be applicable to intact proteins and could enable robust, ...[more]