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Neutron-encoded mass signatures for multiplexed proteome quantification.

ABSTRACT: We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.

SUBMITTER: Hebert AS

PROVIDER: S-EPMC3612390 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Neutron-encoded mass signatures for multiplexed proteome quantification.

Hebert Alexander S AS Merrill Anna E AE Bailey Derek J DJ Still Amelia J AJ Westphall Michael S MS Strieter Eric R ER Pagliarini David J DJ Coon Joshua J JJ

Nature methods 20130224 4

We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed ...[more]

PMID: 23435260

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