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A fast and selective near-infrared fluorescent sensor for multicolor imaging of biological nitroxyl (HNO).

ABSTRACT: The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.

SUBMITTER: Wrobel AT

PROVIDER: S-EPMC3985477 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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A fast and selective near-infrared fluorescent sensor for multicolor imaging of biological nitroxyl (HNO).

Wrobel Alexandra T AT Johnstone Timothy C TC Deliz Liang Alexandria A Lippard Stephen J SJ Rivera-Fuentes Pablo P

Journal of the American Chemical Society 20140313 12

The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live ma ...[more]

PMID: 24564324

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