Ontology highlight
ABSTRACT:
SUBMITTER: Jones AM
PROVIDER: S-EPMC3985517 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Jones Alexander M AM Danielson Jonas Ah JA Manojkumar Shruti N SN Lanquar Viviane V Grossmann Guido G Frommer Wolf B WB
eLife 20140415
Cytosolic hormone levels must be tightly controlled at the level of influx, efflux, synthesis, degradation and compartmentation. To determine ABA dynamics at the single cell level, FRET sensors (ABACUS) covering a range ∼0.2-800 µM were engineered using structure-guided design and a high-throughput screening platform. When expressed in yeast, ABACUS1 detected concentrative ABA uptake mediated by the AIT1/NRT1.2 transporter. Arabidopsis roots expressing ABACUS1-2µ (Kd∼2 µM) and ABACUS1-80µ (Kd∼80 ...[more]