Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.
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ABSTRACT:
SUBMITTER: Vinkenborg JL
PROVIDER: S-EPMC6101214 | biostudies-literature | 2009 Oct
REPOSITORIES: biostudies-literature
Publications
Genetically encoded FRET sensors to monitor intracellular Zn2+ homeostasis.
Vinkenborg Jan L JL Nicolson Tamara J TJ Bellomo Elisa A EA Koay Melissa S MS Rutter Guy A GA Merkx Maarten M
Nature methods 20090830 10
We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found that cytosolic Zn(2+) was buffered at 0.4 nM in pancreatic beta cells, and we found substantially higher Zn(2+) concentrations in insulin-containing secretory vesicles. ...[more]
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