Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics.

ABSTRACT: Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.

SUBMITTER: Lin R 

PROVIDER: S-EPMC8149693 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature