Ontology highlight
ABSTRACT:
SUBMITTER: Deshpande RA
PROVIDER: S-EPMC3989629 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Deshpande Rajashree A RA Williams Gareth J GJ Limbo Oliver O Williams R Scott RS Kuhnlein Jeff J Lee Ji-Hoon JH Classen Scott S Guenther Grant G Russell Paul P Tainer John A JA Paull Tanya T TT
The EMBO journal 20140203 5
The Mre11-Rad50 complex is highly conserved, yet the mechanisms by which Rad50 ATP-driven states regulate the sensing, processing and signaling of DNA double-strand breaks are largely unknown. Here we design structure-based mutations in Pyrococcus furiosus Rad50 to alter protein core plasticity and residues undergoing ATP-driven movements within the catalytic domains. With this strategy we identify Rad50 separation-of-function mutants that either promote or destabilize the ATP-bound state. Cryst ...[more]