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A simple method for the determination of reduction potentials in heme proteins.

ABSTRACT: We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

SUBMITTER: Efimov I

PROVIDER: S-EPMC3999514 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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A simple method for the determination of reduction potentials in heme proteins.

Efimov Igor I Parkin Gary G Millett Elizabeth S ES Glenday Jennifer J Chan Cheuk K CK Weedon Holly H Randhawa Harpreet H Basran Jaswir J Raven Emma L EL

FEBS letters 20140117 5

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins. ...[more]

PMID: 24440354

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