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Metalloenzyme design and engineering through strategic modifications of native protein scaffolds.

ABSTRACT: Metalloenzymes are among the major targets of protein design and engineering efforts aimed at attaining novel and efficient catalysis for biochemical transformation and biomedical applications, due to the diversity of functions imparted by the metallo-cofactors along with the versatility of the protein environment. Naturally evolved protein scaffolds can often serve as robust foundations for sustaining artificial active sites constructed by rational design, directed evolution, or a combination of the two strategies. Accumulated knowledge of structure-function relationship and advancement of tools such as computational algorithms and unnatural amino acids incorporation all contribute to the design of better metalloenzymes with catalytic properties approaching the needs of practical applications.

SUBMITTER: Petrik ID

PROVIDER: S-EPMC4008701 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Metalloenzyme design and engineering through strategic modifications of native protein scaffolds.

Petrik Igor D ID Liu Jing J Lu Yi Y

Current opinion in chemical biology 20140208

Metalloenzymes are among the major targets of protein design and engineering efforts aimed at attaining novel and efficient catalysis for biochemical transformation and biomedical applications, due to the diversity of functions imparted by the metallo-cofactors along with the versatility of the protein environment. Naturally evolved protein scaffolds can often serve as robust foundations for sustaining artificial active sites constructed by rational design, directed evolution, or a combination o ...[more]

PMID: 24513641

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