Ontology highlight
ABSTRACT:
SUBMITTER: Kazrani AA
PROVIDER: S-EPMC4027163 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Kazrani Asgar Abbas AA Kowalska Monika M Czapinska Honorata H Bochtler Matthias M
Nucleic acids research 20140314 9
PvuRts1I is a prototype for a larger family of restriction endonucleases that cleave DNA containing 5-hydroxymethylcytosine (5hmC) or 5-glucosylhydroxymethylcytosine (5ghmC), but not 5-methylcytosine (5mC) or cytosine. Here, we report a crystal structure of the enzyme at 2.35 Å resolution. Although the protein has been crystallized in the absence of DNA, the structure is very informative. It shows that PvuRts1I consists of an N-terminal, atypical PD-(D/E)XK catalytic domain and a C-terminal SRA ...[more]